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Image Search Results
Journal: International Journal of Ophthalmology
Article Title: Promotion of axon regeneration and inhibition of astrocyte activation by alpha A-crystallin on crushed optic nerve
doi: 10.18240/ijo.2016.07.04
Figure Lengend Snippet: A: Immunofluorescence staining showed the GFAP-positive fibers (green) and TUJ1-positive processes (red) in the crush site following ONC. The sections were obtained from the ONC injury group (injury only) (a-d), PBS-treated ONC injury group (PBS injection) (e-h) and αA-crystallin-treated ONC injury group (α-A injection) (i-l). B, C: WB analysis showed that the GFAP levels in the optic nerve were significantly decreased in the αA-crystallin-treated group compared to the injury only (P<0.01) and PBS-treated groups (P<0.01). However, the TUJ1 levels were higher in the αA-crystallin-treated group compared to the injury only group (P<0.05). cr: The middle of crush site; ONC: Optic nerve crush. The quantitative data represent the means±SD (n=5). aP<0.05, bP<0.01. Scale bar=100 µm.
Article Snippet: The sections were then incubated with the following primary antibodies in 3% goat serum overnight at 4°C: rabbit anti-glial fibrillary acidic protein (GFAP) (Abcam, Cambrige, MA, USA, 1:500),
Techniques: Immunofluorescence, Staining, Injection
Journal: Frontiers in Aging Neuroscience
Article Title: Age-Dependent Activation and Neuronal Differentiation of Lgr5+ Basal Cells in Injured Olfactory Epithelium via Notch Signaling Pathway
doi: 10.3389/fnagi.2020.602688
Figure Lengend Snippet: Aging altered neuronal generation in the olfactory epithelium (OE). (A) Immunostaining against mature and immature sensory neuronal markers olfactory marker protein (OMP) and TUJ1 in the OE of young and aged mice at 3- and 25-month old. The positive signals against OMP were labeled by dash lines. (B) Immunostaining against sensory neuronal marker PGP9.5 (noted by asterisks) and columnar-ciliated respiratory epithelial cell marker Krt19 (noted by dash lines). (C–L) Confocal images of staining against OMP and TUJ1 (C,D) , PGP9.5 (E,F) , Krt19 ( G,H , for respiratory epithelial cells, labeled by arrows), Sox2 ( I,J , for apical supporting cells), and Krt14 ( K,L , for HBCs) in the OE of young and aged mice. Nuclei were counterstained with DAPI, as shown in blue. (M) Statistical analysis of OMP+, TUJ1+, Krt19+, Krt14+, and apical Sox2+ cell density in the OE of young and aged mice. In young and aged OE, 1,406 and 723 OMP+, 262 and 179 TUJ1+, 162 and 133 Krt19+, 98 and 139 Krt14+, and 182 and 222 apical Sox2+ cells were counted. (N) Statistical analysis of OE thickness from young and aged mice. Each OE thickness was calculated from 18 sections of three mice. The statistical significance was determined by unpaired t -test with Mann–Whitney correction. ** p = 0.0072, *** p < 0.001 in (M) , * p = 0.3151 in (N) . Scale bars: 0.5 mm in (B) and 10 μm in (L) .
Article Snippet: The primary antibodies used in the immunostaining and their marked cellular subtypes, dilutions, vendors, and category numbers were as follows: rabbit anti-OMP (#ab87338, Abcam, 1:200, mature olfactory sensory neurons),
Techniques: Immunostaining, Marker, Labeling, Staining, MANN-WHITNEY